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proteome profiler human phospho mapk array membranes  (R&D Systems)


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    R&D Systems proteome profiler human phospho mapk array membranes
    Figure 4. STEAP2 Knockdown Decreases JNK and p38 phosphorylation. (a) The results of the phospho- MAPK protein array analysis using STEAP2 knockdown and control cells. Cell lysates were assessed using the <t>Proteome</t> Profiler Human Phospho-MAPK Array membranes <t>(ARY003B,</t> R&D Systems) overnight at 4 °C. Biotinylated detection antibodies were applied, and membranes were visualized using chemiluminescence. (b) The graph shows the pixel intensity of proteins with significant difference in STEAP2 knockdown (KD) cells compared to matched control cells. Densitometry analysis was performed using the Protein Array Analyzer for ImageJ. The decreased phosphorylation patterns of JNK isoforms at T183/Y185 (c) and p38 isoforms at T180/Y182 (d) due to STEAP2 KD were validated by Western blot. Western blot image was cropped to show appropriate protein band (SNU398 blot cropped). GAPDH protein level was used to validate equal sample loading.
    Proteome Profiler Human Phospho Mapk Array Membranes, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 829 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+phospho-mapk+array+(proteome+profiler/Proteome+Profiler+Human+Phospho-Kinase+Array+Kit/pm38830975-244-6-13
    Average 96 stars, based on 829 article reviews
    proteome profiler human phospho mapk array membranes - by Bioz Stars, 2026-09
    96/100 stars

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    1) Product Images from "STEAP2 promotes hepatocellular carcinoma progression via increased copper levels and stress-activated MAP kinase activity."

    Article Title: STEAP2 promotes hepatocellular carcinoma progression via increased copper levels and stress-activated MAP kinase activity.

    Journal: Scientific reports

    doi: 10.1038/s41598-024-63368-2

    Figure 4. STEAP2 Knockdown Decreases JNK and p38 phosphorylation. (a) The results of the phospho- MAPK protein array analysis using STEAP2 knockdown and control cells. Cell lysates were assessed using the Proteome Profiler Human Phospho-MAPK Array membranes (ARY003B, R&D Systems) overnight at 4 °C. Biotinylated detection antibodies were applied, and membranes were visualized using chemiluminescence. (b) The graph shows the pixel intensity of proteins with significant difference in STEAP2 knockdown (KD) cells compared to matched control cells. Densitometry analysis was performed using the Protein Array Analyzer for ImageJ. The decreased phosphorylation patterns of JNK isoforms at T183/Y185 (c) and p38 isoforms at T180/Y182 (d) due to STEAP2 KD were validated by Western blot. Western blot image was cropped to show appropriate protein band (SNU398 blot cropped). GAPDH protein level was used to validate equal sample loading.
    Figure Legend Snippet: Figure 4. STEAP2 Knockdown Decreases JNK and p38 phosphorylation. (a) The results of the phospho- MAPK protein array analysis using STEAP2 knockdown and control cells. Cell lysates were assessed using the Proteome Profiler Human Phospho-MAPK Array membranes (ARY003B, R&D Systems) overnight at 4 °C. Biotinylated detection antibodies were applied, and membranes were visualized using chemiluminescence. (b) The graph shows the pixel intensity of proteins with significant difference in STEAP2 knockdown (KD) cells compared to matched control cells. Densitometry analysis was performed using the Protein Array Analyzer for ImageJ. The decreased phosphorylation patterns of JNK isoforms at T183/Y185 (c) and p38 isoforms at T180/Y182 (d) due to STEAP2 KD were validated by Western blot. Western blot image was cropped to show appropriate protein band (SNU398 blot cropped). GAPDH protein level was used to validate equal sample loading.

    Techniques Used: Knockdown, Phospho-proteomics, Protein Array, Control, Western Blot

    Related Articles

    other:

    Article Title: Turnover of PPP1R15A mRNA encoding GADD34 controls responsiveness and adaptation to cellular stress.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Cell line nucleofector kit T Lonza Cat#VCA-1002 Cell Proliferation Reagent WST-1 Roche Cat#11644807001 Click-iT Nascent RNA Capture kit Invitrogen Cat#C10365 ERCC RNA Spike-In Mix Thermo Fisher Scientific Cat#4456740 Proteome Profiler Human Phospho-MAPK Array R&D Systems Cat#ARY002B NEBNext rRNA Depletion Kit v2 (Human/ Mouse/Rat) New England Biolabs Cat#E7400L Deposited data RNA Seq Huh7 Ctrl and Brf1/TTP DKO cells This paper GEO: GSE261399 Experimental models: Cell lines HEK293T (human embryonic kidney cell line, female) Pear et al.82 RRID: CVCL_0063 Huh7 (hepatocyte-derived carcinoma cell line, male) Nakabayashi et al.83 RRID: CVCL_0336 Huh7 b–globin reporter WT Blr This paper N/A Huh7 b–globin reporter TNFa Blr This paper N/A Huh7 b–globin reporter GADD34 Blr This paper N/A Huh7 b–globin reporter GADD34MUT Blr This paper N/A Huh7 GADD34DARE clones This paper N/A Huh7 TTP KO clones This paper N/A Huh7 Brf1 KO clones This paper N/A Huh7 TTP/Brf1 DKO clones This paper N/A Huh7 neg ctrl clones This paper N/A Huh7 TTP/Brf1/GADD34 TKO clones This paper N/A Huh7 GADD34 KO clones This paper N/A Oligonucleotides See Table S1 N/A Recombinant DNA pCMV D8.91 Prof. Didier Trono Zufferey et al.84 N/A pMD2.G Prof. Didier Trono N/A pcDNA3-7B b-globin reporter GADD34 human 3’UTR This paper N/A pcDNA3-7B b-globin reporter GADD34 human 3’UTR mutated This paper N/A pWPI b-globin reporter WT Blr This paper N/A pWPI b-globin reporter TNFa ARE Blr This paper N/A pWPI b-globin reporter GADD34 human 3’UTR Blr This paper N/A pWPI b-globin reporter GADD34 human 3’UTR mutated Blr This paper N/A pENTR221 ZFP36 cDNA clone human ORFeome Library N/A pENTR221 ZFP36L1 cDNA clone human ORFeome Library N/A pWPI eGFP-myc-his Puro This paper N/A pWPI TTP-myc-his Puro This paper N/A pWPI Brf1-myc-his Puro This paper N/A pWPI msTTP-WT-myc-his Puro This paper N/A pWPI msTTP-m1,2-myc-his Puro This paper N/A pWPI msTTP-AA-myc-his Puro This paper N/A (Continued on next page) 20 Cell Reports 43, 114069, April 23, 2024

    Article Title: GADD34 mRNA turnover controls the molecular memory of cellular stress experience
    Article Snippet: Briefly, 400 μg of protein from mock- and HCV-infected Huh7 cells lysed at 72 h post infection or Huh7 cells treated for 1 h with DMSO or thapsigargin were analyzed using the Proteome Profiler Human Phospho-MAPK Array (R&D Systems) as recommended by the manufacturer.

    Article Title: Turnover of PPP1R15A mRNA encoding GADD34 controls responsiveness and adaptation to cellular stress.
    Article Snippet: Kinase arrays were conducted as described in.59 Briefly, 400 mg of protein frommock and HCV-infected Huh7 cells lysed at 72 h post infection or Huh7 cells treated for 1 h with DMSO or thapsigargin were analyzed using the Proteome Profiler Human Phospho-MAPK Array (R&D Systems) as recommended by the manufacturer.

    Phospho-proteomics:

    Article Title: PBRM1 presents a potential prognostic marker and therapeutic target in duodenal papillary carcinoma
    Article Snippet: Cells were then counted by staining with DAPI solution and images were captured by using Leica SP8 LIGHTNING confocal microscopy. .. The Human Phospho‐MAPK Array Kit (ARY003B, R&D Systems®, Inc. USA) was performed to detect the relative phosphorylation levels of 43 human kinases. .. Chemiluminescence Gel Imaging System (Bio‐Rad, USA) was used to detect the array's chemiluminescence signal (details of the steps are in ).

    Expressing:

    Article Title: Peroxiredoxin 3 regulates breast cancer progression via ERK-mediated MMP-1 expression
    Article Snippet: .. Relative expression levels of phosphorylated MAPK and other serine/threonine kinases were examined using the Proteome Profiler human phospoho-MAPK antibody array kit (R&D Systems). ..

    Article Title: Peroxiredoxin 3 regulates breast cancer progression via ERK-mediated MMP-1 expression.
    Article Snippet: .. Human phospho-MAPK proteome profiler array Relative expression levels of phosphorylated MAPK and other serine/threonine kinases were examined using the Proteome Profiler human phospoho-MAPK antibody array kit (R&D Systems). ..

    Ab Array:

    Article Title: Peroxiredoxin 3 regulates breast cancer progression via ERK-mediated MMP-1 expression
    Article Snippet: .. Relative expression levels of phosphorylated MAPK and other serine/threonine kinases were examined using the Proteome Profiler human phospoho-MAPK antibody array kit (R&D Systems). ..

    Article Title: Peroxiredoxin 3 regulates breast cancer progression via ERK-mediated MMP-1 expression.
    Article Snippet: .. Human phospho-MAPK proteome profiler array Relative expression levels of phosphorylated MAPK and other serine/threonine kinases were examined using the Proteome Profiler human phospoho-MAPK antibody array kit (R&D Systems). ..



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    proteome profiler human phospho mapk array membranes  (R&D Systems)
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    R&D Systems proteome profiler human phospho mapk array membranes
    Figure 4. STEAP2 Knockdown Decreases JNK and p38 phosphorylation. (a) The results of the phospho- MAPK protein array analysis using STEAP2 knockdown and control cells. Cell lysates were assessed using the <t>Proteome</t> Profiler Human Phospho-MAPK Array membranes <t>(ARY003B,</t> R&D Systems) overnight at 4 °C. Biotinylated detection antibodies were applied, and membranes were visualized using chemiluminescence. (b) The graph shows the pixel intensity of proteins with significant difference in STEAP2 knockdown (KD) cells compared to matched control cells. Densitometry analysis was performed using the Protein Array Analyzer for ImageJ. The decreased phosphorylation patterns of JNK isoforms at T183/Y185 (c) and p38 isoforms at T180/Y182 (d) due to STEAP2 KD were validated by Western blot. Western blot image was cropped to show appropriate protein band (SNU398 blot cropped). GAPDH protein level was used to validate equal sample loading.
    Proteome Profiler Human Phospho Mapk Array Membranes, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Figure 4. STEAP2 Knockdown Decreases JNK and p38 phosphorylation. (a) The results of the phospho- MAPK protein array analysis using STEAP2 knockdown and control cells. Cell lysates were assessed using the <t>Proteome</t> Profiler Human Phospho-MAPK Array membranes <t>(ARY003B,</t> R&D Systems) overnight at 4 °C. Biotinylated detection antibodies were applied, and membranes were visualized using chemiluminescence. (b) The graph shows the pixel intensity of proteins with significant difference in STEAP2 knockdown (KD) cells compared to matched control cells. Densitometry analysis was performed using the Protein Array Analyzer for ImageJ. The decreased phosphorylation patterns of JNK isoforms at T183/Y185 (c) and p38 isoforms at T180/Y182 (d) due to STEAP2 KD were validated by Western blot. Western blot image was cropped to show appropriate protein band (SNU398 blot cropped). GAPDH protein level was used to validate equal sample loading.
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    proteome profiler human phospoho mapk antibody array kit  (R&D Systems)
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    PRDX3 regulates MMP1 via MAPK. ( A ) Multi-pathway reporter array showed the transcriptional activities change of AP-1 in PRDX3-OE group (refer to Supplementary Figure for full investigated pathways). Relative luciferase activity was denoted as the ratio of firefly luciferase to renilla luciferase values. ( B ) Representative western blots showing protein expression of p-c-Jun, total c-Jun. Lamin B1 was used as nuclear loading control while GAPDH was used as cytoplasmic fraction control. ( C ) Human phospho-MAPK <t>proteome</t> profiler array demonstrates PRDX3 induced phosphorylation of MAPK and other serine/threonine kinases. Data is representative of a single experiment out of two experiments done. ( D ) Graph bars depicted the relative protein expression levels of several phosphorylated proteins in PRDX3-OE samples. ( E ) Treatment with SCH772984, an ERK inhibitor, for 24 h inhibited MMP1 expression in PRDX3-overexpressing MDA-MB-231 cells. Each bar represents mean ± SEM, ** P < 0.01, *** P < 0.001, **** P < 0.0001
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    human phospho mapk array kit  (R&D Systems)
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    PRDX3 regulates MMP1 via MAPK. ( A ) Multi-pathway reporter array showed the transcriptional activities change of AP-1 in PRDX3-OE group (refer to Supplementary Figure for full investigated pathways). Relative luciferase activity was denoted as the ratio of firefly luciferase to renilla luciferase values. ( B ) Representative western blots showing protein expression of p-c-Jun, total c-Jun. Lamin B1 was used as nuclear loading control while GAPDH was used as cytoplasmic fraction control. ( C ) Human phospho-MAPK <t>proteome</t> profiler array demonstrates PRDX3 induced phosphorylation of MAPK and other serine/threonine kinases. Data is representative of a single experiment out of two experiments done. ( D ) Graph bars depicted the relative protein expression levels of several phosphorylated proteins in PRDX3-OE samples. ( E ) Treatment with SCH772984, an ERK inhibitor, for 24 h inhibited MMP1 expression in PRDX3-overexpressing MDA-MB-231 cells. Each bar represents mean ± SEM, ** P < 0.01, *** P < 0.001, **** P < 0.0001
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    Image Search Results


    Figure 4. STEAP2 Knockdown Decreases JNK and p38 phosphorylation. (a) The results of the phospho- MAPK protein array analysis using STEAP2 knockdown and control cells. Cell lysates were assessed using the Proteome Profiler Human Phospho-MAPK Array membranes (ARY003B, R&D Systems) overnight at 4 °C. Biotinylated detection antibodies were applied, and membranes were visualized using chemiluminescence. (b) The graph shows the pixel intensity of proteins with significant difference in STEAP2 knockdown (KD) cells compared to matched control cells. Densitometry analysis was performed using the Protein Array Analyzer for ImageJ. The decreased phosphorylation patterns of JNK isoforms at T183/Y185 (c) and p38 isoforms at T180/Y182 (d) due to STEAP2 KD were validated by Western blot. Western blot image was cropped to show appropriate protein band (SNU398 blot cropped). GAPDH protein level was used to validate equal sample loading.

    Journal: Scientific reports

    Article Title: STEAP2 promotes hepatocellular carcinoma progression via increased copper levels and stress-activated MAP kinase activity.

    doi: 10.1038/s41598-024-63368-2

    Figure Lengend Snippet: Figure 4. STEAP2 Knockdown Decreases JNK and p38 phosphorylation. (a) The results of the phospho- MAPK protein array analysis using STEAP2 knockdown and control cells. Cell lysates were assessed using the Proteome Profiler Human Phospho-MAPK Array membranes (ARY003B, R&D Systems) overnight at 4 °C. Biotinylated detection antibodies were applied, and membranes were visualized using chemiluminescence. (b) The graph shows the pixel intensity of proteins with significant difference in STEAP2 knockdown (KD) cells compared to matched control cells. Densitometry analysis was performed using the Protein Array Analyzer for ImageJ. The decreased phosphorylation patterns of JNK isoforms at T183/Y185 (c) and p38 isoforms at T180/Y182 (d) due to STEAP2 KD were validated by Western blot. Western blot image was cropped to show appropriate protein band (SNU398 blot cropped). GAPDH protein level was used to validate equal sample loading.

    Article Snippet: Cell lysates were assessed using the Proteome Profiler Human Phospho-MAPK Array membranes (ARY003B, R&D Systems) overnight at 4 °C.

    Techniques: Knockdown, Phospho-proteomics, Protein Array, Control, Western Blot

    PRDX3 regulates MMP1 via MAPK. ( A ) Multi-pathway reporter array showed the transcriptional activities change of AP-1 in PRDX3-OE group (refer to Supplementary Figure for full investigated pathways). Relative luciferase activity was denoted as the ratio of firefly luciferase to renilla luciferase values. ( B ) Representative western blots showing protein expression of p-c-Jun, total c-Jun. Lamin B1 was used as nuclear loading control while GAPDH was used as cytoplasmic fraction control. ( C ) Human phospho-MAPK proteome profiler array demonstrates PRDX3 induced phosphorylation of MAPK and other serine/threonine kinases. Data is representative of a single experiment out of two experiments done. ( D ) Graph bars depicted the relative protein expression levels of several phosphorylated proteins in PRDX3-OE samples. ( E ) Treatment with SCH772984, an ERK inhibitor, for 24 h inhibited MMP1 expression in PRDX3-overexpressing MDA-MB-231 cells. Each bar represents mean ± SEM, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Journal: Cancer Cell International

    Article Title: Peroxiredoxin 3 regulates breast cancer progression via ERK-mediated MMP-1 expression

    doi: 10.1186/s12935-024-03248-x

    Figure Lengend Snippet: PRDX3 regulates MMP1 via MAPK. ( A ) Multi-pathway reporter array showed the transcriptional activities change of AP-1 in PRDX3-OE group (refer to Supplementary Figure for full investigated pathways). Relative luciferase activity was denoted as the ratio of firefly luciferase to renilla luciferase values. ( B ) Representative western blots showing protein expression of p-c-Jun, total c-Jun. Lamin B1 was used as nuclear loading control while GAPDH was used as cytoplasmic fraction control. ( C ) Human phospho-MAPK proteome profiler array demonstrates PRDX3 induced phosphorylation of MAPK and other serine/threonine kinases. Data is representative of a single experiment out of two experiments done. ( D ) Graph bars depicted the relative protein expression levels of several phosphorylated proteins in PRDX3-OE samples. ( E ) Treatment with SCH772984, an ERK inhibitor, for 24 h inhibited MMP1 expression in PRDX3-overexpressing MDA-MB-231 cells. Each bar represents mean ± SEM, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Article Snippet: Relative expression levels of phosphorylated MAPK and other serine/threonine kinases were examined using the Proteome Profiler human phospoho-MAPK antibody array kit (R&D Systems).

    Techniques: Luciferase, Activity Assay, Western Blot, Expressing, Control, Phospho-proteomics